3D атлас лимфатической системы головного мозга


The lumenized Prox-1/Lyve-1-expressing vessels in the human brain.

Figures 1,2 and Fideos 1-4 demonstrate the lumenized Lyve-1/Prox-1- expressing vessels and clearly show distinct walls composed of a single endothelial layer. The lymphatic vessels (LVs) were found in the inferior occipital gyrus. The colocalization with two markers of the lymphatic endothelial cells (LECs) clearly revealed that an expression of the main LECs transcription factor, Prox-1, was indeed delectable with the Lyve-1 protein and with DAPI (Figure 1( 2,5,6,7 ) and 2, Videos 2,3,4). The videos 1,2,4 show the Lyve-1/Prox-1 vessels in 3D projections. The cerebral LVs can be a key player in the communications between the subarachnoid space and the brain parenchyma creating the drainage tunnel for the interstitial fluid outflow into subarachnoid space. Video 6 illistrate a model of brain lymphatic drainage: the LVs are presented along the cerebral blood vessels, express Lyve-1/Prox-1 and have valves, a single endothelial layer, undulating shape of lymphatic endothelial cells. The pulsation of the cerebral arteries is the driving force behind the movement of fluids from the perivascular spaces to the extracellular space. The cerebral lymphatic vessels direct the brain fluids from the perivascular spaces to the subarachnoid space. The immunohistochemical (IHC) assay of brain slices stained for CD31 and Lyve1 revealed that the Lyve1+structures were identified along CD31+ capillaries, arterioles and venules with an enlarged perivascular spaces (Figure 3). The IHC analysis revealed that the CD68+ cells are not part of Lyve-1+ structures, but they are expressed close to each other along CD31+ vessels (Figure 4a). The CD68 cells were identified directly in the lumen of Lyve-1-structures (Figure 4b).


3D атлас лимфатической системы головного мозга реализован благодаря гранту Российского научного фонда № 21-75-10088